Figure 3
From: Activation of the hedgehog pathway in advanced prostate cancer
Detection of HIP in human cancer specimens. By Western blotting, HIP antibodies (R&D systems Cat# AF1568) recognized one band between 75 and100 KD (A). Expression of endogenous HIP was detected in two GI cancer tissues, which were known to contain activated hedgehog signaling (data not shown here), but not in the matched normal tissue (B). Immunohistostaining of HIP I prostate cancer showed a similar pattern to PSA (C, 200×)