Figure 5
From: Mitochondrial inhibition of uracil-DNA glycosylase is not mutagenic
UDG activity in mitochondrial extracts of parental MCF12A cells and transfected cell lines: Lanes 1, 3, 5 and 7 show UDG activity in mitochondrial extracts from MCF12A parental cell line, cells transfected with pCMV UNG, cells transfected with pCMV UGI, cells transfected with pCMV/myc/mito control vector and commercially available UDG enzyme, that acted as a positive control, respectively. Lanes 2, 4, 6, 8 and 10 shows an inhibition of UDG activity when commercially available Ugi was added in mitochondrial extracts from MCF12A parental cell line, cells transfected with pCMV UNG, cells transfected with pCMV UGI, cells transfected with pCMV/myc/mito control vector and commercially available UDG enzyme, that acted as a positive control, respectively.