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Figure 1 | Molecular Cancer

Figure 1

From: The proteosome inhibitor MG132 attenuates Retinoic Acid Receptor trans-activation and enhances trans-repression of Nuclear Factor κB. Potential relevance to chemo-preventive interventions with retinoids

Figure 1

Suppression of NFκB signaling activity allows for the induction of retinoic acid receptor (RAR) message and protein levels and an increase in RAR signaling activity. WT-Line 1 tumor cells and their non-malignant counterparts (mIκB-Line 1) transduced with a dominant negative inhibitor of NFκB were assessed for the expression of RAR transcripts by RT-PCR, using primers specific for RAR subtypes. Exponential amplification of β-actin was utilized as a loading control (A). Western blot analysis for RAR expression in WT and mIκB-Line 1 tumor cells, using a pan-RAR antibody, demonstrates increased RAR protein levels in mIκB-Line 1 tumor cells (B) and correspondingly increased RAR reporter activity (C).

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Molecular Cancer

ISSN: 1476-4598