Figure 5

Effect of inhibition of DNA methylation by 5-aza-CdR and TSA-treatment on SLIT3 promoter in SiHa cell line. Biallelically methylated HIC1 gene was used as control [29]. U, unmethylated primer; M, methylated primer; Note the absence of methylated allele after treatment with 5-aza-CdR, and 5-aza-CdR+TSA of SLIT3 gene (top panel). Note the decreased intensity of methylated allele and reappearance of unmethylated allele of HIC1 after 5-aza-CdR, and 5-aza-CdR+TSA treatments (bottom panel).