Methylation mediated silencing of TMS1/ASC gene in prostate cancer

Table 5 Risk of prostate cancer in relation to gene methylation, total patients and by race.

	Number and (%) of tissues with gene methylation		Age-adjusted
Gene	Prostate Cancer	Control (BPH)	*odds ratio (95% CI)**	P-value
TMS1/ASC	42/66 (63.6)	12/34 (35.3)	3.7 (1.4 – 9.4)	0.008
Blacks	14/21 (66.7)	7/12 (58.3)	1.1 (0.2 – 5.5)	0.914
Whites	28/45 (62.2)	5/22 (22.7)	7.6 (2.1 – 27.3)	0.002
GSTP1	46/66 (69.7)	1/34 (2.9)	69.7 (8.7 – 558.7)	<0.0001
Blacks	15/21 (71.4)	0/12 (0.0)	--
Whites	31/45 (68.9)	1/22 (4.6)	--
CD44	46/66 (69.7)	13/34 (38.2)	2.7 (1.1 – 6.9)	0.033
Blacks	14/21 (66.7)	4/12 (33.3)	2.9 (0.6 – 14.4)	0.190
Whites	32/45 (71.1)	9/22 (40.9)	2.6 (0.9 – 8.1)	0.093
ECAD	39/66 (59.1)	5/34 (14.7)	7.9 (2.6 – 24.0)	<0.001
Blacks	15/21 (71.4)	2/12 (16.7)	10.9 (1.7 – 70.9)	0.013
Whites	24/45 (53.3)	3/22 (13.6)	7.2 (1.8 – 29.2)	0.006
RASSF1A	34/66 (51.5)	6/34 (17.7)	4.1 (1.5 – 11.8)	0.008
Blacks	10/21 (47.6)	1/12 (8.3)	8.6 (0.9 – 84.7)	0.064
Whites	24/45 (53.3)	5/22 (22.7)	3.2 (0.9 – 10.7)	0.056
EBR	49/66 (74.2)	23/34 (67.7)	1.3 (0.5 – 3.5)	0.582
Blacks	15/21 (71.4)	7/12 (58.3)	1.9 (0.4 – 9.5)	0.460
Whites	34/45 (75.6)	16/22 (72.7)	1.1 (0.3 – 3.7)	0.912

* For the overall odds ratio estimate, we modeled the logit of the probability of being a case (p), as a function of methylation in the particular gene and age: ln [p/(1-p)] = b₀ + b₁ × gene + b₂ × Age. For the race-specific age-adjusted odds-ratio estimates, the following model was fitted: ln [p/(1-p)] = b₀ + b₁ × gene + b₂ × Age + b₃ × Race + b₄ × gene × Race. The interaction between TMS1/ASC and race was marginally significant (p = 0.068), while the interactions between each other genes and race were not statistically significant at the 5% level (that is, corresponding p-values were greater than 0.454).

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