Figure 4From: Sensitive detection of human papillomavirus type 16 E7-specific T cells by ELISPOT after multiple in vitro stimulations of CD8+ T cells with peptide-pulsed autologous dendritic cellsRepresentative presentation for each donor of a positive well (B) compared to a control well (A) in the IFN-γ ELISPOT plate. CD8+ T cells stimulated multiple times with HPV-16 E7 peptide-pulsed mature DC were analyzed for antigen-specific IFN-γ secretion by means of IFN-γ ELISPOT. For this analysis cultured CD8+ T cells were stimulated with HPV-16 E7 peptide-pulsed K562-HLA-A*0201 cells (B), while non-pulsed K562-A*0201 cells were used as control cells (A).Back to article page