Figure 1
From: SMC3 knockdown triggers genomic instability and p53-dependent apoptosis in human and zebrafish cells
Cloning and chromosomal mapping of the zebrafish smc3 gene. A) The ORF of the zebrafish smc3 gene was assembled by aligning the sequences of the EST clones identified through a search of the zebrafish genome and dEST databases using the human SMC3 polypeptide sequence as query. Zebrafish smc3 encodes a protein that is >95% identical to the human protein. B) The zebrafish smc3 locus is synthenic to the human SMC3 locus. Genes mapping in close proximity to both the smc3 and SMC3 gene locus are also identified. MXI: max interactor protein 1 (a MYC-binding protein); DUSP5: dual specificity phosophatase 5; SHOC2: suppressor of clear homolog (a RAS-binding protein); ADRA2: alpha2A adrenergic receptor. C) Prediction of the protein coiled-coil domains. The numbers on the abscissa corresponds to the amino acid residues. The probability of the polypeptide to assume a coiled coil conformation is plotted on the ordinate axis. The contribution of the gene's exons to the five-domain structural organization of the protein is illustrated at the bottom of the coiled-coil probability plot.