From: SMC3 knockdown triggers genomic instability and p53-dependent apoptosis in human and zebrafish cells
Treatments | Embryos no. | Apoptotic Phenotype (%) |
---|---|---|
(A) Uninjected | Â | Â |
1 nl Danieau buffer | 60 | 0% |
(B) SMC3 Morpholino-antisense | Â | Â |
smc3-MO (1 ng) | 60 | 45% |
smc3-MO (2 ng) | 60 | 56% |
smc3-MO (4 ng) | 60 | 86% |
smc3-MO (8 ng) | 60 | 100% |
smc3-mmMO (8 ng) | 60 | 5% |
(C) zfSMC3 rescue | Â | Â |
smc3-MO (4 ng)/smc3 mRNA (250 pg) | 60 | 15% |
(D) p53 Morpholino-antisense rescue | Â | Â |
p53-MO (4 ng) | 60 | 0% |
smc3-MO (4 ng)/p53-MO (4 ng) | 60 | 0% |