Skip to main content
Figure 3 | Molecular Cancer

Figure 3

From: Regulation of angiogenesis and invasion by human Pituitary tumor transforming gene (PTTG) through increased expression and secretion of matrix metalloproteinase-2 (MMP-2)

Figure 3

Expression of MMP-2. A: Semi quantitative RT/PCR analysis of MMP-2 mRNA in HEK293 cells transfected with PTTG CDNA or pcDNA3.1 vector. GAPDH was used as an internal control. B: PCR products shown in A were scanned and quantitated. MMP-2 expression was increased by ~22 fold in HEK293 cells transfected with PTTG cDNA compared to cells transfected with pcDNA3.1. C: Measurement of MMP-2 gene promoter activity. HEK293 cells were co-transfected with MMP-2 gene promoter and PTTG cDNA or pcDNA3.1 vector. MMP-2 promoter gene promoter activity was measured by assaying luciferase activity. The data shown represent fold change of MMP-2 promoter activity in HEK293 cells when co-transfected with pGL2-MMP-2 and PTTG cDNA compared to cells co-transfected with pGL2-MMP-2 and pcDNA3.1 vector. Values were normalized with renila luciferase activity used as an internal control and represent as mean ± SEM of three independent experiments performed in triplicate. ** = p < 0.05.

Back to article page

Molecular Cancer

ISSN: 1476-4598