Cell types
|
Colony numbers
a
|
% Tumor incidence (n)
b
|
---|
NHA (parental) | 0 | ND |
NHA/TS | 0 | 0 (3) |
NHA/TSR | 664 ± 19 | 100 (3) |
NHA/TSRA | 736 ± 51 | 100 (3) |
NHA/TSR + MGMT | 649 ± 34 | ND |
NIH3T3/R | 1440 ± 56 | ND |
NIH3T3/R + MGMT | 1344 ± 88 | ND |
- aNHA (2 × 104) and NIH3T3 cells (1 × 104) were plated in soft-agar (0.36% top agar containing 5% fetal calf serum in 60-mm dishes) and incubated for 21 days and 14 days, respectively. Colonies were stained with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, and the numbers of stained colonies were counted. Results represent mean ± SD from two independent triplicate experiments.
- b3 × 106 cells were injected s.c. into nude mice. n, number of animals treated. ND, not done.