ID
|
Age
|
Gender
|
Diagnosis
|
Ki-67 (%)
a
|
p53 mutations
b
|
p53 staining
c
|
---|
G1 | 73 | F | GBM | 4.0 | N | + |
G2 | 58 | M | GBM | 44.3 | R156P, H214R | ++ |
G3 | 36 | M | GBM | 24.8 | N | - |
G4 | 77 | F | GBM | 92.5 | N | - |
G5 | 53 | M | GBM | 12.6 | N | ++ |
G6 | 61 | M | GBM | 43.6 | N | + |
G7 | 83 | M | GBM | 53.1 | H193P | ++ |
G8 | 28 | M | GBM | 29.0 | H214R | +++ |
G9 | 55 | F | AA | 3.3 | N | +++ |
G10 | 42 | F | AE | 42.6 | N | - |
- aMIB-1 labeling index (by anti-Ki-67 antibody) was analyzed using the MetaMorphV7.0 software (Molecular Cevices, Downingtown, PA, USA), by counting 500–2,000 nuclei per case.
- bp53 cDNA was amplified by RT-PCR and the products were directly sequenced. N, no mutation was detected.
- cStaining intensity was classified into four categories: +++, strong; ++, moderate; +, weak; -, negative.