Figure 6From: Characterization of thimet oligopeptidase and neurolysin activities in B16F10-Nex2 tumor cells and their involvement in angiogenesis and tumor growthHPLC analysis of BK degradation by B16F10-Nex2 supernatant. BK (20 μM) was incubated for 1 h at 37°C with recombinant TOP (A) or B16F10-Nex2 supernatant (B) in 50 mM Tris-HCl, pH 7.4. The reaction products were separated by HPLC. Bradykinin fragments were determined by mass spectrometry.Back to article page