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Figure 5 | Molecular Cancer

Figure 5

From: Epidermal growth factor potentiates in vitro metastatic behaviour of human prostate cancer PC-3M cells: involvement of voltage-gated sodium channel

Figure 5

Effect of EGF on sub-cellular distribution of VGSC protein expression, determined by immunocytochemistry, confocal microscopy and digital imaging. VGSC expression was denoted by the fluorescence intensity (arbitrary units; AU) of the immunocytochemical label. (A) Typical profile of VGSC protein expression scanned across the cell. (B) Same as (A) after treatment with EGF (100 ng/ml) for 24 h. (C) Data quantified from profiles shown in (A) and (B) dividing the cellular cross-sections into cytoplasmic/internal ("INT") and plasma membrane ("PM") fractions, as described in the Methods. Each histobar denotes mean ± standard error (n = 18 cells from 3 separate experiments for each condition).

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Molecular Cancer

ISSN: 1476-4598