Figure 4

Time-dependent antiproliferative effect of S7-( S ) on melanoma cells. (A) Eight melanoma cell lines were treated with 50 μM S7-(S) and cell proliferation was estimated at the different points, as described in Material and Methods. Results are expressed as percentage of cell growth and represent the average (± standard deviation) of triplicate cultures performed twice. (B) S7-(S) wash-out assay. Three melanoma cell lines and one short term culture of human fibroblasts were treated with 50 μM S7-(S) for various times (6–12–18–24–48 hours), then washed and incubated with S7-(S) free medium. Cell proliferation was estimated 48 hours after initiation of treatment, as described in Material and Methods. Results are expressed as percentage of cell growth and represent the average (± standard deviation) of triplicate cultures performed twice.