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Figure 2 | Molecular Cancer

Figure 2

From: Synthesis and biologic properties of hydrophilic sapphyrins, a new class of tumor-selective inhibitors of gene expression

Figure 2

Biological effects of sapphyrins on A549 lung cancer cells. A, 5-Bromo-2'-deoxyuridine (BrdU) incorporation after exposure to PCI-2050 at indicated concentrations. The effect of actinomycin D (ActD, 200 ng/mL) treatment is shown for comparison. B, Cells were treated with inhibitors for 24 hours, medium was replaced, and viability measured after an additional 48 hours by propidium iodide staining. C, Cells were treated with sapphyrins for 4 hours, washed, and sapphyrin uptake estimated by cellular fluorescence above 650 nm. D, Cells were treated with PCI-2050 at indicated concentrations for 4 hours, washed, treated with dichlorofluorescin diacetate, and dichlorofluorescein fluorescence (DCF) measured in live-gated cells. Effect of hydrogen peroxide (H2O2, 200 μM), actinomycin D (Act D, 5 μg/mL), and pretreatment with L-buthionein- [S,R]-sulfoximine (BSO, 100 μM) for 24 hours are shown for comparison.

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Molecular Cancer

ISSN: 1476-4598