Figure 2From: Overexpression of phosphoserine aminotransferase PSAT1 stimulates cell growth and increases chemoresistance of colon cancer cellsEffect of PSAT1 overexpression on cell proliferation and tumor xenograft. A, Western blot analysis of PSAT1 expression in SW480-FlpIn and SW480-PSAT1 cell lines using anti-PSAT1 or anti-V5 antibodies. B, Growth curve of SW480-FlpIn and SW480-PSAT1 cultured in low serum medium (1%). The curves are representative of three experiments. C, Representative nude mice, SW480-FlpIn were injected subcutaneously into the left flank and SW480-PSAT1 into the right flank. D, Tumor volume evolution after a subcutaneous xenograft of SW480-FlpIn and SW480-PSAT1 in 7 athymic nude mice. E, In vivo tumorigenic frequency for SW480-FlpIn and SW480-PSAT cell lines. Ratios indicate the number of mice presenting an observable tumor compared with the total number of mice observed. Numbers in parentheses indicate percentages.Back to article page