Figure 2
From: DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines
Methylation analysis of CST6, LCN2, SCNN1A, CDH1, CEACAM6 , and ESR1 among putative hypermethylator and low-frequency methylator cell lines. (A) Representative agarose gels of methylation-specific PCR (MSP) products corresponding to CST6, LCN2, SCNN1A, CDH1, CEACAM6, and ESR1 are shown. U = unmethylated MSP product, M = methylated MSP product. Cell line abbreviations are as follows: 231 = MDA-MB-231, 415 = MDA-MB-415, 435S = MDA-MB-435S, 436 = MDA-MB-436, 453 = MDA-MB-453, and 468 = MDA-MB-468. All other cell lines are designated by their full name. (B) Representative bisulfite sequence analysis for CDH1. Methylated CpGs are designated by closed circles, unmethylated CpGs are designated by open circles for MDA-MB-435S, BT20, and MDA-MB-231 cell lines (5 replicates each). (C) Representative agarose gels of RT-PCR products for CST6, SCNN1A, CDH1, CEACAM6, and ESR1 demonstrating 5-aza induction of gene expression in hypermethylator cell lines. RT-PCR results using cDNA template from untreated (-) and 5-aza treated (+) are shown.