Figure 4
From: DNMT3b overexpression contributes to a hypermethylator phenotype in human breast cancer cell lines
Analysis of DNA methyltransferase enzymes among putative hypermethylator and low-frequency methylator cell lines. Results from triplicate determination of total DNMT activity and individual DNMT protein assays are shown. Hypermethylator cell lines are represented by black bars, low-frequency methylators are represented by cross-hatched bars, and MCF12A cells are represented by a white bar. Error bars represent S.E.M. One unit of DNMT activity or DNMT protein level corresponds to the equivalent amount of activity or protein expressed in MCF12A cells. (A) Total DNMT enzymatic activity; (B) DNMT1 protein; (C), DNMT3a protein; and (D), DNMT3b protein.