Figure 6

Dose-dependent analysis of testosterone-HSA and DHT induced apoptosis in Caco2 cells in the absence or presence of caspase-3 inhibitor. Quantitative APOPercentage apoptosis assay of testosterone-HSA and DHT stimulated Caco2 cells. Cells were exposed to 10^-7 M to 10^-10M testosterone-HSA and DHT respectively in the presence or absence of the caspase-3 inhibitor DEVD-fmk for 24 hours. The pro-apoptoric response was assessed by the APOPercentage apoptosis assay. Cells serum starved for comparable period of time served as a positive control for apoptosis. Bars indicate the mean OD measured at 550 nm ± SE of three independent experiments performed in triplicates, normalized versus the untreated control (serum supplemented cells) and presented as percentage (%) of the untreated serum supplemented control cells taken as 100 (*P < 0.05, N = 3).