Figure 2

IHC analysis of Gleason grade-associated protein expression of PDIA3 and MAP3K5. Summary of PDIA3 and MAP3K5 protein expression quantification in tissue samples. Paraffin tissue sections were stained according to a standard IHC protocol using a staining automate and immunoreactivity of the different Gleason patterns identified in each specimen were scored by an uropathologist according to a 4 point scale (no - 0, weak - 1, intermediate - 2 and strong - 3 staining). For both antigens immunoreactivity was higher in tumors than in benign epithelium in accordance with the gene expression and real-time PCR data. Within the different tumor patterns staining intensity increased from CA3 to CA4 and decreased in the most dedifferentiated CA5 tumor regions. Interestingly, PDIA3 staining intensity in CA 3 regions within GS6 tumors (CA3 CS6) and within GS8 tumors (CA3 GS8) differed significantly, whereas this was not observed with MAP3K5. (B: Benign tissue; CA3: Gleason pattern 3, CA4: Gleason pattern 4, CA5: Gleason pattern 5).