Table 3 qRT-PCR verified differentially expressed genes between GS 6 and GS 8-10
No. | Gene symbol | RZPD ID | IMAGE ID | GO ID | GO term | Microarray | qRT PCR | ||
|---|---|---|---|---|---|---|---|---|---|
q-value (%) | fold change | p-value (%) | fold change | ||||||
1 | ANXA5 | IMAGp998G1518 | 66470 | GO:0006916 | anti-apoptosis | 0.00 | 1.47 | 0.10 | 4.28 |
2 | APLP2 | IMAGp998M03537 | 248618 | GO:0016021 | transmembrane | 0.94 | 1.22 | 6.10 | 2.83 |
3 | COL1A2 | IMAGp998D09597 | 271448 | GO:0001501 | skeletal development | 0.40 | 1.18 | 5.12 | 2.02 |
4 | CRIM1 | IMAGp998K13587 | 267780 | GO:0016021 | transmembrane | 0.00 | 1.20 | 8.46 | 3.23 |
5 | ECHS1 | IMAGp998L12154 | 32898 | GO:0006635 | fatty acid beta-oxidation | 2.43 | 1.18 | 5.48 | 1.89 |
6 | HMGB1 | RZPDp201F0834D | 6067961 | GO:0006915 | apoptosis | 0.00 | 1.40 | 3.25 | 4.68 |
7 | MAP3K5 | IMAGp998O22144 | 28450 | GO:0006915 | apoptosis | 0.17 | 1.35 | 6.94 | 2.98 |
8 | NGFRAP1 | IMAGp998O16794 | 347367 | GO:0007275 | multicellular organismal development | 0.00 | 1.32 | 0.59 | 3.95 |
9 | NPM1 | RZPDp202B129D | 3996837 | GO:0006950 | response to stress | 0.00 | 1.30 | 2.59 | 3.07 |
10 | NUB1 | RZPDp1096A0718D | 6064678 | GO:0006511 | ubiquitin-dependent protein catabolic process | 0.64 | 1.12 | 5.87 | 1.62 |
11 | PDIA3 | RZPDp1096G0216D | 5561830 | GO:0006915 | apoptosis | 1.29 | 1.43 | 3.05 | 2.40 |
12 | PLA2G2A | IMAGp998N13665 | 297804 | GO:0006644 | phospholipid metabolic process | 0.50 | 1.75 | 4.92 | 3.00 |
13 | ROCK1 | RZPDp201C1129D | 5575521 | GO:0006915 | apoptosis | 0.17 | 1.17 | 0.00 | 9.76 |
14 | TEGT | RZPDp1096F101D | 160553 | GO:0006915 | apoptosis | 0.00 | 1.32 | 0.73 | 2.77 |
15 | TMEM16G | IMAGp998D184645 | 1895393 | GO:0016021 | transmembrane | 0.00 | 0.66 | 4.37 | 0.35 |
16 | TMEM69 | IMAGp998I22653 | 293085 | GO:0016021 | transmembrane | 3.78 | 1.08 | 4.56 | 2.77 |
17 | TRAF4 | RZPDp201H0728D | 5541746 | GO:0006915 | apoptosis | 4.29 | 1.26 | 8.58 | 2.59 |
18 | VCAN | RZPDp1096C062D | 201932 | GO:0007155 | cell adhesion | 0.00 | 1.31 | 7.06 | 2.92 |
19 | VCP | IMAGp998O10119 | 123873 | GO:0006915 | apoptosis | 1.29 | 1.18 | 7.88 | 2.75 |
20 | VDAC1 | IMAGp998D08136 | 129751 | GO:0008632 | apoptotic program | 0.00 | 1.51 | 0.30 | 5.41 |