Figure 1
From: Transcriptional activation of the Lats1 tumor suppressor gene in tumors of CUX1 transgenic mice
Scanning ChIP analysis of the LATS1 locus using CUX1 antibodies. (A) Diagram of the LATS1 locus. Exons are numbered and the transcription start site is indicated by an arrow. The positions of primer pairs used in the analysis of chipped DNA are indicated by arrows above the map. Primer sequences are given in Materials & Methods. The positions of p110 and p75 CUX1 consensus binding sites (ATCRAT) are indicated by black triangles below the map. The thick line shows the genomic sequences present on the promoter microarray that was used in the location array analysis. (B) Chromatin from Hs578T cells was immunoprecipitated with the CUX1-861 antibody and analyzed by PCR amplification using primers specific for different regions of the LATS1 locus. Templates for the PCR reactions were 0.1% total input DNA (T) or ChIP-purified DNA (IP).