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Table 6 Primers used for quantitative real-time PCR

From: Gene expression profiling of canine osteosarcoma reveals genes associated with short and long survival times

Gene symbol

Forward and Reverse Primers

Temp (°C)

ANKRD17

FW: 5'-AAGTAGCGCACCACCTTCAC-3'

60.0

 

RW: 5'-CTAGCAGCAAATGGTGGACA-3'

 

MRPS31

FW: 5'-GAATTGGTCCTTGCTTTGGA-3'

60.0

 

RW: 5'-ATCCAGTGGACGAAAGATGG-3'

 

NCOR1

FW: 5'-TCTTCCTCTGCGTTTTCCAT-3'

59.6

 

RW: 5'-GCATCCCAAAAACTTTGGAC-3'

 

MGST1

FW: 5'-CGGACAGATGATAGGGTGG-3'

62.0

 

RW: 5'-GATTTGGCTGGGGAAGG-3'

 

RPS19

FW: 5'-CCTTCCTCAAAAAGTCTGGG-3'

61.0

 

RW: 5'-GTTCTCATCGTAGGGAGCAAG-3'

 

HPRT

FW: 5'-AGCTTGCTGGTGAAAAGGAC-3'

56.0

 

RW: 5'-TTATAGTCAAGGGCATATCC-3'

 
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Molecular Cancer

ISSN: 1476-4598