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Figure 4 | Molecular Cancer

Figure 4

From: Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents

Figure 4

Hypersensitivity of HuH-7 cells specifically towards ICL-agents. (A) Cell proliferation assays to compare the sensitivity of five HCC cell lines towards ICL- and non-ICL-chemotherapeutic agents. HuH-7 cells were hypersensitive towards treatment with any of three ICL-agents as compared to four FA pathway-proficient HCC lines (upper panel: MMC, cisplatin, melphalan), with IC50 ratios ranging from 3.4- to 18.6-fold (arrows, indicated in red), but not towards any of three non-ICL-agents (lower panel: 5-FU, doxorubicin, etoposide). Error bars represent SEM of three independent experiments. (B) Flow cytometric cell cycle profiles of five HCC cell lines 48 h after treatment with MMC at the indicated concentrations. A pronounced G2 arrest (defined here as a cell fraction of >40% in G2 [36]) at low-dose MMC (25 nM) was observed only in HuH-7 cells. (C) Graphic representation of the cell cycle distributions from a representative experiment displaying the fraction of cells in G1, S or G2 at the indicated MMC concentrations for the indicated HCC cell lines. Arrows indicate the lowest dose at which a G2 arrest of more than 40% of the cells was observed.

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Molecular Cancer

ISSN: 1476-4598