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Figure 6 | Molecular Cancer

Figure 6

From: Genetic inactivation of the Fanconi anemia gene FANCC identified in the hepatocellular carcinoma cell line HuH-7 confers sensitivity towards DNA-interstrand crosslinking agents

Figure 6

Screening of 18 HCC tissues for inactivation of FANCC, FANCF or FANCG. (A) Chromatograms displaying the identified heterozygous sequence variants (arrows) in FANCC and FANCG. (B) PCR-based HpaII-restriction assays to determine methylation status of FANCC, FANCF and FANCG in 18 HCC tissues. The corresponding CpG island-containing regions were PCR-amplified before and after digestion using the indicated enzymes: U = undigested, H = HpaII digestion, M = MspI digestion. Universal methylated and unmethylated human DNA (hDNA) was used as controls. No hypermethylation of FANCC, FANCF or FANCG was detected in any of the 18 HCC samples. (C) RT-PCR to assess mRNA-expression of FANCC, FANCF and FANCG in 18 HCC tissues. All samples expressed FANCC-, FANCF- and FANCG- mRNA.

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Molecular Cancer

ISSN: 1476-4598