Table 4 AGR2 ELISA performance characteristics
Recombinant AGR2 Protein (ng/ml) | Human Serum | Panc. Cell Lysates | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
40 | 13.3 | 4.44 | 1.48 | 0.494 | 0.165 | 0.055 | CFPAC1-1 | MiaPaca | |||
All Assays | Mean (ng/ml) | 40.30 | 13.20 | 4.54 | 1.41 | 0.48 | 0.18 | 0.07 | 22.00 | 120.70 | 14.60 |
ratio (signal/noise) | 3559 | 1169 | 401 | 124 | 42 | 15 | 6.2 | 195 | 1065 | 129 | |
Intra-assay | SD (ng/ml) | 1.260 | 0.199 | 0.079 | 0.062 | 0.014 | 0.008 | 0.004 | 1.400 | 8.300 | 0.790 |
CV (%) | 3.1 | 1.5 | 1.8 | 4.4 | 2.9 | 4.6 | 5.9 | 6.2 | 6.8 | 5.4 | |
Inter-assay | SD (ng/ml) | 1.240 | 0.207 | 0.081 | 0.067 | 0.012 | 0.008 | 0.006 | 3.400 | 5.100 | 1.000 |
CV (%) | 3.1 | 1.6 | 1.8 | 4.8 | 2.6 | 4.5 | 9.1 | 16.0 | 4.3 | 6.8 | |