Figure 6

Knockdown of NF-κB1 suppresses cell growth and colony formation. (A) In pSilencer/si-NF-κB1 (si-NF-κB1) transfected MGC803 cells, the expression level of NF-κB1 protein was significantly decreased. (B) MGC803 cells were transfected with si-NF-κB1 or control vector. Relative cell growth activity was determined by MTT assay at the indicated times. Values are means ± SD of three duplications and the relative cell growth activity is shown (*P < 0.05). (C) Cell independent growth activity was detected by colony formation assay. MGC803 cells were transfected with si-NF-κB1 or control vector at a final concentration of 5 ng/μl (final concentration) and then seeded in 12-well plates. The number of colonies was counted on the 9th day after seeding. The colony formation rate was calculated and is shown (**P < 0.01). (D) The cells of colony formation assay were stained by crystal violet and the representative pictures of colonies are shown.