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Figure 6 | Molecular Cancer

Figure 6

From: Tumor suppressor FLCN inhibits tumorigenesis of a FLCN-null renal cancer cell line and regulates expression of key molecules in TGF-β signaling

Figure 6

Higher levels of secreted TGF-β2 and activin A proteins were detected in FLCN -restored UOK257-2 cells compared to the parental UOK257 cell line. (A) TGF-β2 and (B) activin A levels in cultured media were measured by ELISA (R&D systems). (C) TGF-β2 induced anchorage independent growth of UOK257 cells. However, (D) activin A suppressed anchorage independent growth of UOK257 cells. UOK257 cells (2,500 cells) were plated in soft-agar and cultured for 4 weeks in the presence of TGF-β2 or Activin A and stained with crystal violet. N = 4 for each treatment.

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Molecular Cancer

ISSN: 1476-4598