Figure 3From: The haematopoietic GTPase RhoH modulates IL3 signalling through regulation of STAT activity and IL3 receptor expressionRhoH upregulates p21 Cip1 and p27 Kip1 expression. (A) Quantification of p21Cip1 and p27Kip1 mRNA expression levels by quantitative SYBR-green PCR in control cells, RhoH and siRhoH-transduced cells. Data were normalised to mRNA levels detected in control cells compared to GAPDH. Statistical significance was analysed using the 's t-test (mean ± SD, n = 3; *P ≤ 0.05). (B, C) Whole cell lysates of control cells, siRhoH and RhoH expressing cells were separated by 4-20% gradient SDS-PAGE and protein expression was detected by antibodies against p21Cip1 and p27Kip1. Protein levels were normalised to β-actin expression (lower panel). Quantification and statistical significance were performed using the 's t-test (mean ± SD, n = 2; *P ≤ 0.05).Back to article page