Figure 1
From: Regulation of Erk1/2 activation by osteopontin in PC3 human prostate cancer cells
OPN induces Erk1/2 activation. Lysates from PC3 and PC3/OPN cell lines were immunoblotted with phospho-specific antibodies to OPN, Erk1/2, JNK, and p-38 (A-D, upper panels) proteins. Immunoblotting with the corresponding non-phosphorylated antibodies demonstrates equal loading of indicated proteins (A-D, lower panels). Confocal microscopy analysis of distribution of actin (red) and phospho-Erk1/2 (green) in PC3 and PC3/OPN cell lines is shown in Panel E. Results shown are representative of three independent experiments.