Figure 6

Differentially expressed genes in GRP78-knockdown HN-CICs. (A) A total of 79 significantly differentially expressed genes mapped in the human PPIs were clustered (by row) according to their similarities in GRP78- knockdown HN-CICs, red indicating induction and blue indicating repression. (B) Mapped human PPIs among the differentially expressed genes were grouped according to the topological characteristics as highlighted in border colors (periphery: gray; inter-modular hubs: red; and intra-modular hubs: blue). Color legends were according to expression patterns: as for nodes, red - induction and green - repression; as for edges, gray -negatively correlated and orange-positively correlated. Thickness of edges was proportional to the absolute value of PCC and numbers indicated databases reporting such interactions. (C) Top 4 functional annotation clusters analyzed from DAVID were listed. (D) Total proteins were prepared from Sh-Luc and Sh-GRP78 expressing HN-CICs and analyzed by immunoblotting with antibodies against Caspase-3, BAX, PTEN, MAPK or GAPDH as indicated. The amount of GAPDH protein of different crude cell extracts was referred as loading control.