Figure 3
Regulation of CD133, CD44 and ERα mRNA by exogenous or autocrine IL-6. RT-PCR analysis of CD133, CD44, ERα mRNA level in: a) MCF-7 cells in presence/absence of IL-6 (10 ng/ml, 48 h) or 5azadC (15 μM, 48 h), b) p53 D cells and MDA-MB231 cells in presence/absence of αIL-6 (1.5 μg/ml, 48 h) or transfected with scr/siIL-6 (1 μg, 48 h). β-Actin of panel a) and b) are reported in panels 2a and 2b, respectively.