Figure 3
From: Blocking of ERK1 and ERK2 sensitizes human mesothelioma cells to doxorubicin
Inhibition of ERK1 or ERK2 results in more accumulation of Dox in HMESO cells. A. HMESO cells stably transfected with either shERK1 or shERK2 and shControl cells were treated with 0.5 or 5 μM of Dox for 1 or 5 h, and intracellular accumulation of Dox was measured by flow cytometry as described in 'Materials and Methods'. shERK1 or shERK2 HMESO lines show enhanced accumulation of Dox as compared to shControl lines. *p ≤ 0.05 as compared to respective shControl (shCon). N = 2 per group. B. Fluorescence microscopic representation of dose-related Dox accumulation (orange) in stably transfected HMESO cells after 1 h of treatment. All images are at 400X magnification (Bar = 50 μm).