Figure 2
From: Upregulation of FOXM1 induces genomic instability in human epidermal keratinocytes
Upregulation of FOXM1B sensitises cells to UVB-induced apoptosis. (A) FOXM1B upregulation preferentially activated p21, p38, p53 and increase PARP cleavage in primary NHEK following UVB exposure compared to EGFP controls. Immunoblots of EGFP-FOXM1B (using GFP antibody; EGFP-FOXM1B at ~130 kD and not shown are the EGFP bands which run at ~27kD), phospho-p38 MAPK, phospho-p53 (Ser 15), p21, PARP and GAPDH on primary NHEK transduced with either EGFP (GFP) or EGFP-FOXM1B (FOX). Protein lystates were harvested from cells at time 0 (control un-irradiated), 3, 6 and 24 hours following UVB irradiation as indicated. (B) Digital densitometry graphical representations of data in (A). (C) UVB irradiated FOXM1B-overexpressed cells showed a significant *(P < 0.05) 2.4-fold (5.8% in EGFP cells vs 14% in FOXM1B cells) increased in Sub-G1 population. This result is a representative of 3 independent experiments performed in different occasions using different primary NHEK cells.