Cell cycle-dependent regulation of the bi-directional overlapping promoter of human BRCA2/ZAR2 genes in breast cancer cells

Table 1 Sequences of the oligonucleotides used in this study.

Oligo Name	Sequence (5'-3')	Purpose	Product size
P1	TCAGCGAGAAGAGAACAC	BRCA2/ZAR2 gene promoter amplification	497 bp
P2	TTGGCAGAGACAAAAGGGC
P3	CGACTGGAGCACGAGGACACTGA	Gene racer ZAR2 5'-RACE	814 bp
P4	GCTGTTTGTGCCCTGAGAGTCC
P5	AAGCTTATGGAGCGCTTTGTCCGT	PCR of C-terminal FLAG-tagged ZAR2	975 bp
P6	GGATCCCATCACATATTTAAAGCTGTAAA
P7	AAGCTTATGGAGCGCTTTGTCCGT	PCR of N-terminal FLAG-tagged ZAR2	978 bp
P8	TCTAGATCACATCACATATTTAAAGCTGTAAAT
P9	CACTGTAAAGATTGTAAGACCAGG	ZAR2 RT-PCR	140 bp
P10	CTGACATTGGATTGCTTCTACTCG
P11	GTACAGGAAACAAGCTTCTGA	BRCA2 RT-PCR	271 bp
P12	GACTAACAGGTGGAGGTAAAG
P13	GCTCGTCGTCGACAACGGCTC	Beta-actin RT-PCR	352 bp
P14	CAAACATGATCTGGGTCATCTTCTC
P15	ACCCUUACAAGAGGGCGCAGCUUAA	siRNA#1
P16	UUAAGCUGCGCCCUCUUGUAAGGGU
P17	UCCUGUGGCAAUAUUUACAGCUUUA	siRNA#2
P18	UAAA GCUGUAAAUAUUGCCACAGGA
P19	ACCACAUGAGAGCGGCGACUUCUAA	Control siRNA#1
P20	UUAGAAGUCGCCGCUCUCAUGUGGU
P21	UCCCGGUAUAAAUUUCGACUGUUUA	Control siRNA#2
P22	UAAACAGUCGAAAUUUAUACCGGGA
P23	ATCACTACTTGTCATCGTCATCCTTGTAGTCG	FLAG reverse primer

ISSN: 1476-4598