Figure 5

Secreted enzymatically active ATX promoted Hep3B cell invasion. A. a, Hep3B cells were transfected with ATX siRNA or negative siRNA, and ATX expression was examined by immunoblot. b, serum starved Hep3B cells were chemoattracted by MEM/0.1% fatty acid-free BSA or conditioned media (CM) plus or minus 1 μM LPC (18:1). Neg. CM, CM from Hep3B cells transfected with negative siRNA. 3B CM, CM from Hep3B cells; ATX siRNA CM, CM from Hep3B cells transfected with ATX siRNA. Each sample was tested in triplicate and results are reported as mean ± SD of invaded cells of two independent experiments, bars, SD. *, P < 0.05. c, representative fields of invaded and stained cells. B. a, starved Hep3B cells were chemoattracted by various doses of LPA and representative fields of invaded cells are shown in b. **, P < 0.01.