Figure 5

TNFα is able to sensitize NBL cell lines to cisplatin- and etoposide-induced cell death. A. FasL mRNA levels were assessed by qRT-PCR in cisplatin- (30 μM) and etoposide- (30 μM) treated SK-N-AS cells for the indicated times. mRNA levels were normalized using 18S mRNA. B. Caspase-8 cleavage was analyzed by Western blot in SK-N-AS cells pre-treated with 100 ng/ml TNFα for 24 h after cisplatin (30 μM) and etoposide (30 μM) treatments. C. Cell death assay by Hoechst staining in the indicated cell lines pre-treated with 100 ng/ml TNFα for 24 h and treated with the indicated concentrations of cisplatin (Cis) and etoposide (Eto) for 48 h. *p ≤ 0.05; **p ≤ 0.01; *** p ≤ 0.001.