Fig. 1

HGF and TGFβ1 differently affected Twist and Snail in 1833 cells. We show representative images of Western blots, performed with nuclear (a and b) and cytosol (c) protein extracts from treated cells, and all the experiments were repeated three times with similar results. The samples were run on gel, and were processed under the same experimental conditions. B23, a marker of the nuclear fraction, and vinculin, a cytosolic protein, were used for normalization. The densitometric evaluation of protein bands was performed; when multiple bands for a protein were present, they were considered together in the densitometric evaluation. The numbers at the bottom of the Western blots indicate the fold variations for pTwist1/Twist and pWwox/Wwox ratios after HGF or TGFβ1 treatment versus the corresponding value of starved cells, considered as 1. The graphics in a and c show the changes of protein levels after the treatments, and the data are the means ± S.E. of three independent experiments. Where the S.E. bars are not shown, they lie in the symbol. *P < 0.05, **P < 0.005 versus the starvation value (Δ)