Fig. 5

Downstream signaling targets of IMB-R1. Western blot analysis of signaling targets in serum-starved (SS) cells (48 h) released into FGF2 in the absence (a) or presence (b) of IMB-R1 for the time points indicated. a, Following SS, cells were stimulated with FGF2 for 10 min and protein expression determined. b, Following SS, cells were pre-treated with IMB-R1 (1:250 dilution) for 1, 2, 6 or 24 h then stimulated with 20 ng/ml (MG63) or 5 ng/ml (T47D) of FGF2 for 10 min and protein expression determined. c, Cells were dosed with U0126 for 1 h and phosphorylated-ERKs detected by immunoblotting. d, Cells were treated with U0126 for 24 h and stained with a combination of Annexin V-FITC and PI and presented as per Figure 4 b. e, Cells were treated with IMB-R1 (1:250 dilution) for 48 h and the protein expression determined by immunoblotting