Fig. 1

NFKB1 and RELA are up-regulated in GC cell lines and primary gastric tumors. a The mRNA expression of NFKB1 and RELA in nine GC cell lines compared with GES-1 cells (immortalized gastric epithelium cell line). The standard deviations (SDs) were achieved by qRT-PCR (−Delta Delta Ct values) in triplicate wells. b Western blot analysis of NFKB1 and RELA in nine GC cell lines, GES-1 cells and three normal gastric tissues (Normal 1–3 protein samples are from normal gastric mucosa obtained from weight reduction gastric surgery). c NFKB1 and RELA mRNA expression in 28 paired primary GC samples (NFKB1, P = 0.124; RELA, P = 0.188). d NFKB1 and RELA showed increased protein expression in primary gastric tumors compared with paired non-tumours adjacent tissues (n = 28; NFKB1, P = 0.004; RELA, P = 0.012). e Representative immunohistochemistry images of positive NFKB1 and RELA expression in GC tissue microarray (original magnification × 100, insertion × 400). NFKB1 and RELA expression are mainly localized in the cytoplasm. f Kaplan-Meier plots of disease specific survival according to NFKB1 or RELA expression status. RELA accumulation in cytoplasm was associated with poor disease specific survival in patients with GC (P = 0.045), but NFKB1 overexpression only correlated with a non-significant trend of poor survival (P = 0.274). g The prognosis plots according to NFKB1 and RELA mRNA expression in GC (from KM plotter). Only high RELA mRNA expression significantly correlated with overall survival in 876 GC samples (P < 0.001)