Fig. 1

RT-PCR amplification and sequencing analysis of fusion gene Rad51C-ATXN7. Colorectal tumor (T) RNA isolated by TRIzol method, was RT-PCR amplified and sequenced. The primers (Additional file 1: Table S1) that span exon-5 of Rad51C and exon -8 of ATXN7 produced two fragments of approximately 376 bp and 316 bp (a). The RT-PCR amplified product from a colorectal tumor (T) showed 376 bp fragment only which was absent in the corresponding non-tumor (NT) sample (b). The RT-PCR amplified products were Topo TA cloned and sequenced. The RT-PCR products contained a Variant 1 of Rad51C (exon 1–7) and ATXN7 (exon 6–13) fusion gene (c) and a Variant 2 without exon-7 Rad51C (exon 1–6) ATXN7 (exon 6–13) (d). The solid bar line indicates the sequence breakpoint joining Rad51C and ATXN7