Fig. 2

Ovarian cancer ascites and CCL18 enhance the migration of tumor cells. The migration of ovarian cancer CaOV3 cells (a) and OVCAR3 cells (b) was evaluated at 24 h using Boyden chambers with either serum-free medium (control) or different ovarian cancer ascites as chemoattractants. CaOV3 cells (c) and OVCAR3 cells (d) were treated with 0–50 ng/ml CCL18 for 24 h and migration was determined. Results shown are representative of three independent experiments and data are expressed as fold increased relative to control. P values are indicated relative to controls. e CCL18 levels in ascites were correlated with the ability of ascites to stimulate CaOV3 cell migration. The correlation coefficient (r) was determined by Pearson’s correlation coefficient test. f Incubation of ascites (OVC439 and OVC551) with CCL18-blocking antibody (20 μg/ml) reduces the migration expressed as the number of migrated cells in CaOV3 and OVCAR3 cells. Values as mean ± SEM and represent three independent experiments: *P < 0.001. g Immunoblot analysis of PITPNM3 expression in CaOV3 and OVCAR3 cells