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Fig. 4 | Molecular Cancer

Fig. 4

From: Esculetin induces antiproliferative and apoptotic response in pancreatic cancer cells by directly binding to KEAP1

Fig. 4

Esculetin lowers ROS levels: a Fluorescence intensity of DCFDA, an indicator of ROS level was determined in PANC-1 cells and it was found to decrease upon 100 μM esculetin treatment. b Flow cytometric analysis based histogram showing increase in percentage of PANC-1 cells negative for ROS activity as a function of time of ecsuletin treatment. c Western blot analysis showing temporal decrease in protein levels of ROS dependent transcription factor NK-κB and uniform levels of its inhibitor I-κB in PANC-1 cells. (VC stands for vehicle control, E stands for esculetin treated sample for indicated time, and numerals represent time of esculetin treatment). Data represents the mean ± SD of three independent experiments. The significance was determined using ANOVA (Bonferroni’s test). Key:*p < 0.05; **p < 0.01; ***p < 0.001; **** p < 0.0001)

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Molecular Cancer

ISSN: 1476-4598