Fig. 1

Hyperactivation of STAT3, p-STAT3 and EZH2 was associated with poor survival in the GC cohort. a Differential expression of STAT3 and EZH2 mRNA was detected in GC cells using RT-PCR. b Differential expression of STAT3, p-STAT3 and EZH2 in GC cells using Western Blotting. c Co-expression of STAT3 status and EZH2 protein was detected in GC and matched normal tissues using Western Blotting; d High or low levels of STAT3 status and EZH2 expression in GC and adjacent normal tissues using IHC staining; p-STAT3 and EZH2 showed a focal or diffuse pattern in the nuclei (200 × magnification). Kaplan-Meier analyses show the effect of STAT3 (e), EZH2 (f), p-STAT3 expression (g), or the combination between STAT3 and EZH2 expression (h) in overall survival