Fig. 7

a miR-30a-5p inhibits tumor growth by targeted CD73 in vivo. At the experimental endpoint, tumors treated with the miR-30a-5p agomir were dissected and photographed as indicated. Tumor growth of A549 xenografts in nude mice treated with the miR-30a-5p agomir and NC agomir (n = 3). The graph shows the tumor growth curves at the time of sacrifice with respect to the first measurements, after the administration of 2 nmol miR-30a-5p agomir or NC agomir per mouse seven times every 4 days; the arrows indicate the weight of the excised tumors (mean ± SD, n = 3). b Each tumor formed by the indicated cells was weighed. c and d qRT-PCR analysis of miR-30a-5p levels and CD73 mRNA expression in excised tumors transfected with the miR-30a-5p agomir and NC agomir; U6 and β-actin were used as internal controls, respectively. e Hematoxylin and eosin (H & E) staining confirmed the presence of tumor cells in slices of the indicated tumor sections. Immunohistochemical staining for CD73 and EGFR was quantified based on staining intensity. ^* P < 0.05; ^** P < 0.01