Fig. 2
From: Interplay between CCR7 and Notch1 axes promotes stemness in MMTV-PyMT mammary cancer cells
CCR7 activates the Notch signaling pathway in mammary cancer stem-like cells. a-b Hes1 expression was assessed in primary mammary CSCs from PyMT-Ccr7 WT and Ccr7 −/− tumors. a Hes1 mRNA levels relative to PyMT-Ccr7 −/− in the sorted CD24+CD29hi stem cell-enriched population (left) and in secondary mammospheres (right), n = 4–6 mice/genotype/experiment. b Hes1 protein expression in the CD24+CD29hi sorted cell population, n = 4 mice/genotype. c Relative Hes1 mRNA levels in PyMT-Ccr7 WT secondary mammospheres cultured with and without CCL19 and CCL21. d Relative Hes1 mRNA levels in PyMT-Ccr7 −/− secondary mammospheres cultured with and without CCL19. c–d n = 6 mice/experiment