Fig. 4

Dclk1 knockdown reduced the expression of Dclk1 and Dclk1⁺ cells and the associated expression of tumor stem cell markers and pro-survival signaling in the Apc ^Min/+ mice. a IHC for Dclk1 in the small intestines of Apc ^Min/+ mice treated with siDclk1-NPs and siScramble-NPs; staining intensity was scored and represented as a bar graph. b FACS data representing the % Dclk1⁺ cells isolated from the small intestines of Apc ^Min/+ mice treated with siDclk1-NPs compared with siScramble-NPs. c & d mRNA and protein expression levels of Dclk1, Bmi1, Lgr5, and Musashi1 in the isolated IECs of WT and Apc ^Min/+ mice treated with siDclk1-NPs and siScramble-NPs, analyzed by RT-PCR and western blot. f Protein expression levels of pro-survival signaling and their downstream targets in the isolated IECs of WT and Apc ^Min/+ mice, analyzed by western blot. e mRNA expression levels of pro-survival signaling and their downstream targets in the isolated IECs of WT and Apc ^Min/+ mice, analyzed by RT-PCR. All quantitative data are expressed as means ± SD of a minimum of three independent experiments. P values of <0.05 = *, <0.01 = **, and 0.001 = *** were considered statistically significant