Fig. 6

RASSF8 modulates the NF-κB transcriptional activity and p65 expression. a The cells were co-transfected with a NF-κB luciferase reporter plasmid and RASSF8 overexpressing plasmid or RASSF8 siRNA, and subjected to luciferase assay to detect the transcriptional activity of NF-κB. *P < 0.05. b The cells transfected with RASSF8-overexpressing plasmid or RASSF8 siRNA were exposed to Western blot to determine the expression of nuclear p65 and cytoplasmic p65 expression levels. LaminB1 was used as an internal control to normalize nuclear p65 expression and GAPDH was used as an internal control to normalize cytoplasmic p65 expression