Fig. 3
From: MicroRNA-29c functions as a tumor suppressor by targeting VEGFA in lung adenocarcinoma
MiR-29c modulates LAD cells through targeting VEGFA. a The VEGFA 3’-UTR containing the wildtype or mutant miR-29c binding sequence was inserted into downstream of the luciferase reporter vector. The mutated sequences are in italic. b The dual luciferase reporter assay revealed that the luciferase activity controlled by VEGFA 3’-UTR was inhibited by ectopic miR-29c expression in A549 cells. ***p < 0.001. c VEGFA mRNA levels determined by qRT-PCR following treatment of A549 cells with miR-29c mimic or Anip973 cells with miR-29c inhibitor. **p < 0.01. d ELISA assay of secreted VEGFA protein levels in TCM after treatment of A549 cells with miR-29c mimic or Anip973 cells with miR-29c inhibitor. *p < 0.05, **p < 0.01. e Western blot analyses of VEGFA protein levels following treatment of A549 cells with miR-29c mimic or Anip973 cells with miR-29c inhibitor. β-actin was used as control