Fig. 7
Suppression of tumor formation by glutamine deprivation and L-Asparaginase pretreatment. a A549 cells were pre-treated with glutamine-free medium or L-asparaginase (1 U/ml) for 5 days, then equal numbers of viable cells from the control and experimental groups were inoculated subcutaneously into both flanks of athymic mice at the cell number of 5 × 104 cells/injection site (group 1) or 1 × 104cells/injection site (group 2). The mice were then monitored for tumor incidence (b) and tumor sizes for group1 (c). No tumor formation was observed in cells pre-treated with glutamine-free medium or L-asparaginase in group 2. d Body weight curves of mice inoculated with A549 cells as described in A (group 1)